An optimized protocol for microarray validation by quantitative PCR using amplified amino allyl labeled RNA

BMC Genomics

Table 4 Correlation between microarrays and qPCR

		STAT3	ANXA1	ELANE	LY96	TNF-α-1	TNF-α-2
Microarrays vs original RT protocol	R²	0.72	0.84	0.94	0.68	NA	-0.09
	P	0.002	0.0001	0.0002	0.005	NA	0.77
Microarrays vs optimized RT protocol	R²	0.69	0.84	0.94	0.73	0.11	0.07
	P	0.005	0.0001	0.0001	0.002	0.70	0.80

This table gathers correlation coefficients (R²) and P values estimated by Pearson product moment correlation from experiments shown in Figure 8. See legend to this figure for details. NA: not applicable.

ISSN: 1471-2164