Endotoxin stimulation of TLR15, IL8, IL1B, IFNG, IL6 and IL10 gene expression in HD11 cells. HD11 macrophages were stimulated for 1, 2, 4 and 8 hours with 0.0, 0.1, 1.0, 10.0 μg/ml endotoxin. Data are shown as the fold change in mRNA levels after treatment compared with nontreated cells by QPCR. A: fold change at 1 h post-stimulation (hps); B: fold change at 2 hps; C: fold change at 4 hps; D: fold change at 8 hps. RNA samples were isolated on 3 different days and the QPCR was carried out in triplate.