Table 3 Primers for qPCR
From: Response of swine spleen to Streptococcus suis infection revealed by transcription analysis
| primer |
Accession number | Sequence |
Product size |
|---|---|---|---|
| Plau | NM_213945 |
Forward: CGAACTGTGGCTGTCT Reverse: AGCAGGTTTGCGATGTG | 126 bp |
| S100A9 a | BI402402 |
Forward: CCAGGATGTGGTTTATGGCTTTC Reverse: CGGACCAAATGTCGCAGA | 186 bp |
| S100A12 a | CB475695 |
Forward: GGCATTATGACACCCTTATC Reverse: GTCACCAGGACCACGAAT | 169 bp |
| Hsp70 | NM_213766 |
Forward: AGGCGGAGAAGTACAAAGCG Reverse: GATGGGGTTACACACCTGCTC | 257 bp |
| Timp1 | NM_213857 |
Forward: CGCCTCGTACCAGCGTTAT Reverse: GTGGAAGTATCCGCAGACGC | 127 bp |
| SOD2 | NM_214127 |
Forward: TCTGGACAAATCTGAGCCCT Reverse: GACGGATACAGCGGTCAACTT | 119 bp |
| Il6 b | AF493992 |
Forward: GACAAAGCCACCACCCCTAA Reverse: CTCGTTCTGTGACTGCAGCTTATC | 69 bp |
| Sele | NM_214268 |
Forward: GGATTTGAACTCATCGGACCT Reverse: CATTCTGAGGATGGCCGAC | 115 bp |
| Il1b b | NM_214055 |
Forward: GGCCGCCAAGATATAACTGA Reverse: GGACCTCTGGGTATGGCTTTC | 70 bp |
| Nramp1 | U55068 |
Forward: CGTGGTGACAGGCAAGGACT Reverse: TAGCCGTGCCGATGACTTC | 131 bp |
| Hsp90 | CF180819 |
Forward: CCCAGTTGATGTCGTTG Reverse: CCGTCAGGCTTTCGTAT | 117 bp |
| Il8 b | NM_213867 |
Forward: TTCGATGCCAGTGCATAAATA Reverse: CTGTACAACCTTCTGCACCCA | 176 bp |
| CCL19 | BX672579 |
Forward: GCTAAGCCTCTGGACT Reverse: AATGAGCAGGTAGCGA | 121 bp |
| Haptocorrin | CB472702 |
Forward: ATTCTCAGGGAGTATTCCGTCT Reverse: CTTTGGGGACAAGTAGCAGTT | 105 bp |
| Alox15 | NM_213931 |
Forward: ACCGAGGGTTTCCTGTCT Reverse: AGGTGGTTGGAGGAGTGC | 100 bp |
| TLR2 b | NM_213761 |
Forward:TCACTTGTCTAACTTATCATCCTCTTG Reverse: TCAGCGAAGGTGTCATTATTGC | 162 bp |
| GAPDH b | AF017079 |
Forward: TGCCAACGTGTCGGTTGT Reverse: TGTCATCATATTTGGCAGGTTTCT | 62 bp |