Table 3 Primers for qPCR
From: Response of swine spleen to Streptococcus suis infection revealed by transcription analysis
primer | Accession number | Sequence | Product size |
|---|---|---|---|
Plau | NM_213945 | Forward: CGAACTGTGGCTGTCT Reverse: AGCAGGTTTGCGATGTG | 126 bp |
S100A9 a | BI402402 | Forward: CCAGGATGTGGTTTATGGCTTTC Reverse: CGGACCAAATGTCGCAGA | 186 bp |
S100A12 a | CB475695 | Forward: GGCATTATGACACCCTTATC Reverse: GTCACCAGGACCACGAAT | 169 bp |
Hsp70 | NM_213766 | Forward: AGGCGGAGAAGTACAAAGCG Reverse: GATGGGGTTACACACCTGCTC | 257 bp |
Timp1 | NM_213857 | Forward: CGCCTCGTACCAGCGTTAT Reverse: GTGGAAGTATCCGCAGACGC | 127 bp |
SOD2 | NM_214127 | Forward: TCTGGACAAATCTGAGCCCT Reverse: GACGGATACAGCGGTCAACTT | 119 bp |
Il6 b | AF493992 | Forward: GACAAAGCCACCACCCCTAA Reverse: CTCGTTCTGTGACTGCAGCTTATC | 69 bp |
Sele | NM_214268 | Forward: GGATTTGAACTCATCGGACCT Reverse: CATTCTGAGGATGGCCGAC | 115 bp |
Il1b b | NM_214055 | Forward: GGCCGCCAAGATATAACTGA Reverse: GGACCTCTGGGTATGGCTTTC | 70 bp |
Nramp1 | U55068 | Forward: CGTGGTGACAGGCAAGGACT Reverse: TAGCCGTGCCGATGACTTC | 131 bp |
Hsp90 | CF180819 | Forward: CCCAGTTGATGTCGTTG Reverse: CCGTCAGGCTTTCGTAT | 117 bp |
Il8 b | NM_213867 | Forward: TTCGATGCCAGTGCATAAATA Reverse: CTGTACAACCTTCTGCACCCA | 176 bp |
CCL19 | BX672579 | Forward: GCTAAGCCTCTGGACT Reverse: AATGAGCAGGTAGCGA | 121 bp |
Haptocorrin | CB472702 | Forward: ATTCTCAGGGAGTATTCCGTCT Reverse: CTTTGGGGACAAGTAGCAGTT | 105 bp |
Alox15 | NM_213931 | Forward: ACCGAGGGTTTCCTGTCT Reverse: AGGTGGTTGGAGGAGTGC | 100 bp |
TLR2 b | NM_213761 | Forward:TCACTTGTCTAACTTATCATCCTCTTG Reverse: TCAGCGAAGGTGTCATTATTGC | 162 bp |
GAPDH b | AF017079 | Forward: TGCCAACGTGTCGGTTGT Reverse: TGTCATCATATTTGGCAGGTTTCT | 62 bp |