Complementary evolution of 5' regulatory regions in duplicate F3'5'H s, within 2,000 bp upstream of the translation start site. Red boxes in panels a and b indicate conserved regions, which were aligned using the DiAlign2 algorithm implemented in GEvo, and linked in this figure by pink connectors between the two paralogues. Regulatory DNA stretches uniquely present in either duplicate are represented as horizontal dotted lines. Putative DNA binding motifs were identified using PlantCARE, and are shown in panel a if they occurred in regulatory modules conserved between the two duplicate genes or in panel b if they occurred in regions that distinguished either duplicate. The scale of bp distance from the translation start site and the legend for twelve categories of DNA binding sites are given in panel c.