Table 2 Strains and plasmids used in this study
From: Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling
name | characterization | reference |
|---|---|---|
XL2-blue | E. coli: recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac, [F', proA B, laclqZ ΔM15Tn10(Tetr), Amy, Camr] | Stratagene |
DH5α | E. coli: deoR, endA1, gyrA96, hsdR17(rk-mk+),recA1, relA1, supE44, λlthi-1, Δ(lacZYA-argFV169) | [59] |
EGD | L. monocytogenes Sv 1/2a, wildtype | S. H. E. Kaufmann |
Pkp1 | L. monocytogenes Sv 1/2a, ΔplcA/hly/mpl/actA/plcB, Kanr | [60] |
EGDΔlmo0135-137 | in-frame deletion mutant of a putative oligonucleotide transporter gene | This study |
EGDΔispE | in-frame deletion mutant of lmo0190 involved in mevalonate biosynthesis | This study |
EGDΔlmo0618 | in-frame deletion mutant of a protein kinase gene | This study |
EGDΔlmo1031-1036 | In-frame deletion mutant of an operon responsible for glycerol metabolism | This study |
EGDΔlmo1085 | in-frame deletion mutant of lmo1085 involved in teichoic synthesis | This study |
EGDΔtagB | in-frame deletion mutant of tagB (lmo1088) involved in teichoic synthesis | This study |
EGDΔlmo1506 | in-frame deletion mutant of a putative transporter gene | This study |
EGDΔoppF | in-frame deletion mutant of the putative oligopeptide ABC transporter gene lmo2192 | This study |
EGDΔlmo2734 | in-frame deletion mutant of lmo2734 encoding a putative sugar hydrolase | This study |
EGDΔlmo2781 | in-frame deletion mutant of lmo2781 involved in cellobiose metabolism | This study |
pLSV101 | Temperature-sensitive shuttle vector; EmR | [12] |
EGDΔpdxK | in-frame deletion mutant of pdxK (lmo0662) involved in biosynthesis of pyridoxine | This study |