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Table 2 Strains and plasmids used in this study

From: Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling

name characterization reference
XL2-blue E. coli: recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac, [F', proA B, laclqZ ΔM15Tn10(Tetr), Amy, Camr] Stratagene
DH5α E. coli: deoR, endA1, gyrA96, hsdR17(rk-mk+),recA1, relA1, supE44, λlthi-1, Δ(lacZYA-argFV169) [59]
EGD L. monocytogenes Sv 1/2a, wildtype S. H. E. Kaufmann
Pkp1 L. monocytogenes Sv 1/2a, ΔplcA/hly/mpl/actA/plcB, Kanr [60]
EGDΔlmo0135-137 in-frame deletion mutant of a putative oligonucleotide transporter gene This study
EGDΔispE in-frame deletion mutant of lmo0190 involved in mevalonate biosynthesis This study
EGDΔlmo0618 in-frame deletion mutant of a protein kinase gene This study
EGDΔlmo1031-1036 In-frame deletion mutant of an operon responsible for glycerol metabolism This study
EGDΔlmo1085 in-frame deletion mutant of lmo1085 involved in teichoic synthesis This study
EGDΔtagB in-frame deletion mutant of tagB (lmo1088) involved in teichoic synthesis This study
EGDΔlmo1506 in-frame deletion mutant of a putative transporter gene This study
EGDΔoppF in-frame deletion mutant of the putative oligopeptide ABC transporter gene lmo2192 This study
EGDΔlmo2734 in-frame deletion mutant of lmo2734 encoding a putative sugar hydrolase This study
EGDΔlmo2781 in-frame deletion mutant of lmo2781 involved in cellobiose metabolism This study
pLSV101 Temperature-sensitive shuttle vector; EmR [12]
EGDΔpdxK in-frame deletion mutant of pdxK (lmo0662) involved in biosynthesis of pyridoxine This study