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Table 5 Unigene clusters candidate to encode for miRNAs

From: A computational-based update on microRNAs and their targets in barley (Hordeum vulgare L.)

  Features of the precursors identified Best scoring alignment with miRBase precursors
Unigene cluster ΔG (kcal/mol) MFEI NM ML PL Arm Accession number miRNA Score e-value
Hv.1306 -72.8 0.89 2 21 167 3' MI0006178 tae-MIR444 736 1 × 10-54
Hv.5064 -22.0 1.00 2 18 68 3' MI0006199 tae-MIR1137 168 4 × 10-8
Hv.7117 -74.9 0.96 3 21 115 5' MI0011565 bdi-MIR397 196 5 × 10-10
Hv.8158 -60.7 0.88 3 21 92 5' MI0001763 sof-MIR168a 393 1 × 10-26
Hv.14657 -31.5 1.85 2 21 69 3' MI0006183 tae-MIR1121 241 3 × 10-14
Hv.15131 -51.1 0.90 2 21 129 3' MI0006976 osa-MIR444d 431 2 × 10-29
Hv.16635 -91.7 0.92 3 21 200 3' MI0006170 tae-MIR159a 805 2 × 10-60
Hv.22601 -34.0 0.92 4 22 97 3' MI0006192 tae-MIR1130 179 9 × 10-9
Hv.28058 -63.8 1.60 2 24 129 3' MI0006182 tae-MIR1120 147 7 × 10-6
Hv.29065 -53.6 1.07 4 22 131 5' MI0006199 tae-MIR1137 182 8 × 10-9
Hv.29519 -42.9 1.02 2 21 96 3' MI0006192 tae-MIR1130 144 8 × 10-6
Hv.30469 -39.0 0.91 3 21 117 5' MI0006199 tae-MIR1137 141 2 × 10-5
  1. For each cluster, the table shows details about the putative precursors: the free energy ΔG, the minimal folding free energy index (MFEI), the number of mismatches in miRNA/miRNA* duplex (NM), the mature length (ML), the precursor length (PL) and the location of mature miRNA (3' or 5'). Moreover, it is also reported the more similar known precursor in miRBase, with the alignment score and p-value.