Table 5 Oligonucleotides used in this study
From: Identification of small RNAs in Francisella tularensis
Oligonucleotide | Sequence (5' to 3' direction)a | Use |
|---|---|---|
3' adapter | P--rUrUrUCGGGCCGCGGACTGTidT | cDNA cloning and RACE |
5' adapter | GATATGCGCGAATTCCTGTAGAACGAACACTAGGGGrArArA | cDNA cloning and RACE |
5'PRIMER | GTAGAACGAACACTAGGGGAAA | cDNA cloning and RACE |
3'PRIMER | GACAGTCCGCGGCCCGAAA | cDNA cloning and RACE |
3' adapter_B | P-rUrUrUCTATCCATGGACTGTidT | cDNA cloning tmRNA |
5' adapter_B | GATATGCGCGAATTCCTGTAGAACGAACACTAGAAGrArArA | cDNA cloning tmRNA |
5'PRIMER_B | GTAGAACGAACACTAGAAGAAA | cDNA cloning tmRNA |
3'PRIMER_B | TACAGTCCATGGATAGAAA | cDNA cloning tmRNA |
ftrA_GSP_5'RACE | GTTATTCAGACGTGTCAAACAGAG | 5'-RACE ftrA |
ftrA_GSP_3'RACE | GTACCAAATAATTAATGCTCTGTAATC | 3'-RACE ftrA |
ftrB_GSP_5'RACE | GAGATTCCCGCCTACGCGG | 5'-RACE ftrB |
ftrB_GSP_3'RACE | GATACTAACTTAACGTCGGTAGTC | 3' RACE ftrB |
ftrA_DelR | GCGCGGCCGC GTGGTAAAATCATCTAGGTTCTAGC | Deletion ftrA |
ftrA_DelB | CATTTATAATTTTAGATATTTTTTCGC | Deletion ftrA |
ftrA_DelO | GCGAAAAAATATCTAAAATTATAAATG GGCAATTAATATATCTTGTTCGCTTCTTAGC | Deletion ftrA |
ftrA_DelT | GCGTCGAC GCAACTAAGAAAAGAATATTTAATAGCC | Deletion ftrA |
ftrA_DelCheck1 | CATATGTAGTGTACTTTATTTAAATAC | Verification of ftrA deletion |
ftrA_DelCheck2 | CCTAAGTTTCAGTTGCTGAATTATTTGG | Verification of ftrA deletion |
ftrA_DelCheck3 | GCCACTGAAGGCGGAAATCTCGC | Verification of ftrA deletion |
ftrA_DelCheck4 | CAGTTAAATATTATTAACATTAAGAAAC | Verification of ftrA deletion |
ftrB_DelR | GCGCGGCCGC CTTTTAAGATTTGTATTCTTATTTGTTC | Deletion ftrB |
ftrB_DelB | CACTACCCCGTATTGCTTCGCAAGCC | Deletion ftrB |
ftrB_DelO | GGCTTGCGAAGCAATACGGGGTAGTGCCTAAGGAGTCAAACTAACAAAGGGGCCTGC | Deletion ftrB |
ftrB_DelT | GCGTCGAC CAGAGCATTTATGATAGTTTGTTTTCC | Deletion ftrB |
ftrB_DelCheck1 | CTAAATCTAAGGAATGATAATTAACC | Verification of ftrB deletion |
ftrB_DelCheck2 | GGACAGGAATGGACAGCAGAAG | Verification of ftrB deletion |
ftrB_DelCheck3 | GTATATCCTATTTGAAAAGCTAATGGC | Verification of ftrB deletion |
ftrB_DelCheck4 | CACTATATGGATATGCTTATGAACAAGC | Verification of ftrB deletion |
ProbeA | CAGACGTGTCAAACAGAGGTCCGTTCAAAATAC | Northern blot |
ProbeB | GAGATTCCCGCCTACGCGGGAATGACTACCGACG | Northern blot |
FTL_0044_F | GCTATATGTCCCAGGTGTAAGG | qRT-PCR |
FTL_0044_R | GCTCTTTGGCTTTTTTAGGGGTC | qRT-PCR |
FTL_0421_F | GGGCAACTGTAACAGTTAAGC | qRT-PCR |
FTL_0421_R | CTTCTTTGTCATAAACTACATTAGC | qRT-PCR |
FTL_0836_F | GTGGCTATTGATGACATACTCAAC | qRT-PCR |
FTL_0836_R | GCTAAGCCTAGATAACTGATACC | qRT-PCR |
FTL_1922_F | GGATTTGATTTTTCTCCAATTATTG | qRT-PCR |
FTL_1922_R | CTGCGCAATAATGCTTTGTATG | qRT-PCR |