Figure 1

Characterization of the Chromosome 5p15.1 TAF11-Like macrosatellite array. (A) Ideogram of chromosome 5 and schematic map of the region surrounding the macrosatellite. Right facing arrow heads represent the array. BASP1 is indicated to the left. Scale indicates the approximate distances represented on the map in kb. Below the Array is a map of an individual 3.4 kb monomer (Large right facing arrow). BglII restriction endonuclease sites that define a single monomer are indicated to the left and right. Repetitive elements LTR, Alu and Charlie are indicted by the shaded and patched boxes. The ORF for TAF11-Like is highlighted and orientation indicated by the left facing arrow. (B) Higher-order organization of the chromosome 5 array as revealed by dot-plot analysis. On the y-axis is a 3.4 kb monomer and on the x-axis is the 147 kb genomic interval (17.5 Mb according to build hg18). XbaI restriction endonuclease sites that immediately flank the array that are used for the PFGE are indicated. Dot-plot generated using NCBI Blast, and output image labelled in Adobe Photoshop CS2. (C) Copy number variation of the chromosome 5 macrosatellite array. Southern blot of XbaI cut DNA from 22 unrelated individuals separated by pulsed field gel electrophoresis and hybridized with a chromosome 5 macrosatellite probe. The ethnicity of the individuals is indicated at the top. The size of the hybridizing fragments in kb are indicated on the blot. Arrows to the left indicate the range in size of the array as inferred copy number.