Figure 4

PITG_02700: Trypsin-like serine protease. A) Multiple sequence alignment showing the sequence similarity between PITG_02700 and its paralogs and well-characterized human and Aedes homologs. Regions spanning the catalytic triad (indicated by *) are shown. Proteins belonging to the P. infestans secretome are labeled with a signal peptide (SigP.) icon. GIP1, Glucanase Inhibitor Protein 1. B) Position of PITG_02700 and other P. infestans trypsin-like serine proteases on the FIR heat map (Figure 2B). C) in planta expression pattern of three in planta-induced GIP-like genes (left) and three other secreted serine protease genes (right), including PITG_02700. Expression of the effector gene Avr3a is given as a reference. Dpi, days post inoculation.