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Table 1 Oak (Q. petraea and Q. robur) cDNA libraries for Sanger sequencing

From: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak

Species Library code Library name Library type Kit for library construction No. of genotypes Tissue Sample stage/treatment
Q. petraea A LG0BAC Standard LambdaZAP 50 bud Quiescent buds from 2-year-old trees (Phalsbourg (57-F) and Mirecourt (88-F)) sampled in April 7th and 9th, 2004
  B QpBudslate Standard CloneMiner 2 bud Early swelling bud sampled in March 24th and 30th , 2006 on adult trees
  C QpSwellingBud Subtractive SMART PCR, BD PCR-Select, pCR4 TOPO 20 bud Swelling vs. quiescent buds, 1-year-old trees
  D QpBudquiescent Subtractive SMART PCR, BD PCR-Select, pCR4 TOPO 60 bud Developing (internodes have started to grow) vs. quiescent buds, 1-year-old trees
  E QpVegetativeGrowth Subtractive SMART PCR, BD PCR-Select, pCR4 TOPO 20 bud Quiescent vs. swelling buds, 1-year-old trees
  F sessile48hours Subtractive SMART PCR, BD PCR-Select, pGEM-T easy 10 root Hypoxia for 24 and 48 h. White roots from 6-month-old cuttings, 2005
  G sessile6 hours Subtractive SMART PCR, BD PCR-Select, pGEM-T easy 10 root Hypoxia for 6 h. White roots from 6-month-old cuttings, 2005
  H Qp5stressRoots Standard CloneMiner 15 root 6-month-old seedlings collected in October 2006, 1/10°C 3 days, 2/35°C 4 days, 3/CO2 700 ppm, 4/water stress, 5/hypoxie 48 h
  I QpLeaf5stress Standard CloneMiner 15 leaf 6-month-old seedlings collected in October 2006 : 1/10°C 3 days, 2/35°C 4 days, 3/CO2 700 ppm, 4/water stress, 5/hypoxie 48 h
  J QpXyleme Standard Creator SMART 2 xylem Secondary differentiating xylem sampled in May 21th, 2007 on adult trees
Q. robur K QrBudsEarly Standard CloneMiner 3 bud Setting bud sampled in October 26th, 2006 on adult trees
  L QrBudslate Standard CloneMiner 3 bud Swelling buds sampled in March 24th and 30th, 2007 on adult trees
  M LG00BAD Standard LambdaZAP 10 root Fine roots under optimal fertilization and Irrigation conditions, harvested in August 2004 [48]
  N pedonculate6 hours Subtractive SMART PCR, BD PCR-Select, pGEM-T easy 10 root Hypoxia for 6 h. White roots from 6-month-old cuttings, 2005
  O pedonculate48 hours Subtractive SMART PCR, BD PCR-Select, pGEM-T easy 10 root Hypoxia for 24 and 48 h. White roots from 6-month-old cuttings, 2005
  P LG0BAA Standard LambdaZAP 3 leaf Young leaves sampled on adult trees in April 27th, 2004
  Q HighWUE Subtractive SMART PCR, BD PCR-Select, pGEM-T easy 5 leaf Green leaves on one-year-old cuttings, (high vs. low WUE) October 2005
  R LowWUE Subtractive SMART PCR, BD PCR-Select, pGEM-T easy 5 leaf Green leaves on one-year-old cuttings, (low vs. high WUE) October 2005
  S LG0BAB Standard LambdaZAP 3 xylem Secondary differentiating xylem sampled on adult trees in April 27th, 2004
  T QrAnoxie Standard Creator SMART 10 root Hypoxia for 24 and 48 h. White roots from 6-month-old cuttings, 2005