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Table 4 Oak (Q. petraea and Q. robur) cDNA libraries for 454-pyrosequencing

From: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak

Species

Library code

Library name

Library type

Kit for library construction

No. of genotypes

Tissue

Sample stage/treatment

Q. petraea

I

LC1-EcoEndoDorm

Standard

SMART PCR cDNA Synthesis Kit

30

Buds

Endodormancy, sampled in September 17th and 24th and October 1st, 2005

Q. petraea

II

LC2-EcoEndoDorm

Standard

SMART PCR cDNA Synthesis Kit

30

Buds

Ecodormancy, sampled in January 14th and 28th and February 11th, 2005

Q. petraea

III

SJ1-EcoEndoDorm

Standard

SMART PCR cDNA Synthesis Kit

30

Buds

Endodormancy, sampled in September 17th and 24th and October 1st, 2005

Q. petraea

IV

SJ2-EcoEndoDorm

Standard

SMART PCR cDNA Synthesis Kit

30

Buds

Ecodormancy, sampled in January 14th and 28th and February 11th, 2005

Q. petraea

V

10QS-Intersp

Standard

SMART PCR cDNA Synthesis Kit

10

Leaves, buds

Young and mature leaves, quiescent and later buds

Q. robur

VI

10QP-intersp

Standard

SMART PCR cDNA Synthesis Kit

10

Leaves, buds

Young and mature leaves, quiescent and later buds

Q. petraea

VII

FS

Standard

SMART PCR cDNA Synthesis Kit

2

Flower

Pollen, flowers

Q. robur

VIII

FP

Standard

SMART PCR cDNA Synthesis Kit

2

Flower

Pollen, flowers

Q. petraea

IX

Qs21

Normalized

MINT cDNA synthtesis Kit,

1

Leaves, buds

Quiescent, swelling buds; young, mature leaves

Q. petraea

X

Qs28

Normalized

MINT cDNA synthtesis Kit,

1

Leaves, buds

Quiescent, swelling buds; young, mature leaves

Q. petraea

XI

Qs29

Normalized

MINT cDNA synthtesis Kit,

1

Leaves, buds

Quiescent, swelling buds; young, mature leaves

Q. robur

XII

3P

Normalized

MINT cDNA synthtesis Kit,

1

Leaves, buds

Quiescent, swelling buds; young, mature leaves

Q. robur

XIII

11P

Normalized

MINT cDNA synthtesis Kit,

1

Leaves, buds

Quiescent, swelling buds; young, mature leaves

Q. robur

XIV

A04

Normalized

MINT cDNA synthtesis Kit,

1

Leaves, buds

Quiescent, swelling buds; young, mature leaves