Figure 5

Expression of NYFA mRNA. (a) Diagrammatic representation of the NFYA genomic structure in relation to the poly-Q encoding region traversing the NFYA splice site across exons 2 and 3. The PCR amplicons corresponding to the exon 2-intron 2 and intron 2-exon 3 boundary regions when using genomic DNA (gDNA), and the amplicon produced when using cDNA as template are shown as thick black lines. The sequence at each splice boundary is shown with exonic sequence in black and intronic sequence in red. The absolutely conserved intronic sequence elements at the splice sites are underlined. (b) PCR amplicon profiles for four individuals. The left hand and right hand panels show the exon 2 - intron 2 and intron 3 - exon 3 amplicons, respectively, with genomic DNA as template. The central panel shows the amplicons produced using mammary tissue cDNA and primers traversing the splice site junction. Two amplicons encoding 4 or 5 Q were identified. (c) Tissue specific NYFA mRNA splicing across the poly-Q encoding region. Representative results are shown for one of four individuals for four different tissues.