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Figure 2 | BMC Genomics

Figure 2

From: Evaluation of external RNA controls for the standardisation of gene expression biomarker measurements

Figure 2

Linear range of detection on microarray and RT-qPCR platforms. Example plots of modelling the linear range of each gene expression platform are displayed. Normalised signal intensities (microarrays) (symbol: black diamond) or ΔCt values (RT-qPCR) (symbol: grey triangle) are plotted against the standard (copy number/ng). Individual data points for ERCC-13 from three independent experiments are plotted. Correlation analysis was performed over the linear portion of the detection range (Microarrays: 10-105 copies/ng; RT-qPCR 10 - 106 copies/ng) and Pearson correlation coefficient (R2) values are displayed (microarray value in bold).

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