Figure 7

Characterization of the suppressive sites affecting the activity of the mouse CRELD2 and ALG12 promoters. Neuro2a cells were transfected with the indicated CRELD2 or ALG12 reporter constructs. Twenty-four hours after transfection, the cells were incubated with or without Tg (0.1 μM) for 10 h, and the luciferase activity was measured (A, B and C). Each deleted suppressive site in the CRELD2 and ALG12 promoters is shown as site I, II or III. The ERSE motif and two NF-Y binding sites in the mouse CRELD2 and ALG12 reporters are shown with a box and underlines, respectively. The nucleotide sequence of the each mutated NF-Y binding site is shown with small letters (B and C). Control and Tg-treated activities are shown as open and closed columns, respectively. Values represent means ± SD from 3 independent cultures and are expressed relative to the basal activity of the pGL3-Basic vector.