Profiling N-acetylation in yeast. A Venn diagram representation of the overlap between identified N-acetylated proteins carrying the N-acetylation on position 1 or 2, and protein variants detected to display N-acetylation on amino acid position 3 or higher. Sequence logos were calculated for peptides acetylated at position 1 and 2 from the predicted gene-start. For position 1, sequences were devided into peptides that matched the NatB consensus sequence and the rest which is most likely acetylated by other N-acetyltransferases such as NatC. Acetylation in position 2 was found to follow the consensus sequence of NatA. Frequency logos are displayed at the top in the blue frames. The frequency logo for proteins N-acetylated on a amino acid higher than 2 (from the predicted gene-start) are indicated below in the grey panel, revealing no particular consensus sequence for this latter category. Network analysis was performed on these latter protein variants and the three main protein clusters are indicated below the frequency logo. These protein variants were found to be preferentially involved in the proteasome, chaperone network and energy metabolism. Proteins detected to be N-acetylated either in position 1 or 2 and additionally at a position higher than 2 are indicated in blue in the protein clusters. All detected protein variants are given in Additional file 5.