Conservation of NatB substrates across species. Panel A displays a bar chart indicating the conservation of NatB targets across species. This analysis was performed using either all 59 identified NatB substrates (black bars) or only the 5 most conserved proteins Arp2p, Bos1p, Erg3p, Rpb5p and Rps28ap (gray bars). NatB substrates are only sporadically conserved in the tree of life with the exception of a few, highly conserved, proteins. The phylogenetic relationship between the species included in this survey is indicated on the left. Panel B shows an alignment of Glc7p with orthologous protein sequences from different species of the fungal kingdom indicating general high conservation at the full-length protein level. The termini, however, are much less conserved including the part that determines N-acetyltransferase substrate specificity.