Figure 5

MHC class I graph-cluster. The MHC class I graph-cluster (cluster 05625) that contains allelic variation, alternative spliced transcripts and potential gene duplication(s). A) The nodes of the network cluster represent contigs, solid lines (edges) represent reads split between the two contigs during assembly by NEWBLER, and the dotted lines represent homologous contigs representing either divergent alleles from either the same or duplication genes. The orange contigs were identified as homologues of MHC class I in squamates (lizards and snakes), the light blue contigs had no hits in NCBI-NR. B) Alignment of the contigs to their BlastX hit in the NCBI-NR database, MHC class I antigen from Iguanid lizard, Conolophus subcristatus. Again, dotted lines represent homologous contigs representing either divergent alleles from either the same or duplication genes.