Figure 3From: Transcriptome analyses based on genetic screens for Pax3 myogenic targets in the mouse embryoLbx1 expression depends on Pax3. (A, B, E, F) Whole mount in situ hybridisation with an Lbx1 probe of control (A, E), Pax3Pax3-En/+ partial loss of function (B) and Pax3PAX3-FKHR/+ gain of function embryos (F) at E9.25 (A, B) and E10.5 (E, F), showing down-regulation of Lbx1 transcripts when Pax3 activity is reduced, and some up-regulation of its expression in the presence of PAX3-FKHR in somites. In the lower panels (C, D) X-gal staining of β-galactosidase from the IresnlacZ reporter in Pax3IresnlacZ/+ (C) or Pax3Pax3-En-IresnlacZ/+ (D) shows the extent of the somites, notably the hypaxial domain.Back to article page