Figure 6

Microscopic images of altered cell morphology after efficient knock-down of the indicated genes. A - Cells at eight days post infection with the indicated shRNA expression constructs. While in the case of NUP62-A we observed the typical apoptosis phenotype of MDA-MB-231, in the cases of BIRC5-A and HSPA8-A the morphology was very different. BIRC5-knock-down cells were several times larger than cells expressing the NSC, and HSPA8 knock-down cells displayed a fibroblast-like phenotype. NSC, non silencing control. Magnification is 10x. B - Sixteen days post infection cells expressing HSPA8-A were strongly impaired in their viability (see Figure 5B).