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Table 1 Scheme of dilution series of pGIPZ shRNA expression plasmids

From: Decoding pooled RNAi screens by means of barcode tiling arrays

 

Reference pool

Test pool 1

Test pool 2

Test pool 3

Subpool-0

1

1

1

1

Subpool-1

1

9e-01

4e-01

2e-03

Subpool-2

1

1e-03

8e-01

3e-01

Subpool-3

1

1e-04

2e-01

7e-01

Subpool-4

1

1e-01

1e-05

6e-01

Subpool-5

1

5e-01

1e-02

1e-06

Subpool-6

1

4e-03

4e-02

2e-04

  1. Rows show the dilution factors of each of the seven shRNA expression construct subpools in each of the four template pools used for PCR amplification via half hairpin primers or barcode primers respectively. Subpool-0 consists of 245 different pGIPZ plasmids; while subpools-1 to -6 consist of ten different pGIPZ plasmids each.