Figure 4

Primers designed to characterize the genome organizational patterns of ATCC 3502* and UMASS strains. This is part of exploded view of figure 3 on which the primer design is based. This region spans the boundary between block 3 and 4 (ATCC 3502 and ATCC 19397) and the boundary between block 3 and 2 (Hall). Puo, upstream outside primers; Pui, upstream inside primers; P4di, downstream inside primers for block 4; P4do, downstream outside primers for block 4; P2di, downstream inside primers for block 2, and P2do, downstream outside primers for block 2. Arrows with numbers in red indicate the starting (upstream primer) or ending (downstream primers) positions in each genome. Numbers in white inside the blocks indicate the block numbers.