Figure 4

Validation of selected ncRNA candidates. Positive results of snRNA candidate: (1), selected box H/ACA snoRNA candidates: (2)-(5), box C/D snoRNA candidates: (6)-(8), 18mer motif for group1 TCs: (9) and selected group3 TCs: (10)-(14) (E: embryos of 12-18 h; S2: S2 cells). 2% agarose/formaldehyde gels with 25 micrograms of RNA from D. melanogaster's embryo and S2 cells were used for (1), (2), (3), (10), (11) and (12), and 10% denaturing polyacrylamide gels with 25 micrograms of RNA from either embryos or S2 cells were used for the rest. The estimated sizes of bands are listed in Table 4. The left-most lanes of each of groups show the size standards. The estimated size of ~130 nt for the positive bands in lanes (4) and (5) is shown by the arrowhead. (1): snRNA_01; (2):snoHACA_01; (3):snoHACA_05; (4):snoHACA_04; (5): snoHACA_06; (6):snoCD_05; (7):snoCD_24; (8):snoCD_02; (9):ncRNA_01; (10):ncRNA_38; (11):ncRNA_39; (12):ncRNA_50; (13):ncRNA_47; (14):ncRNA_54.