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Table 2 Restriction/Modification products comparison between strains PG2 and 5632

From: Comparative genomic and proteomic analyses of two Mycoplasma agalactiae strains: clues to the macro- and micro-events that are shaping mycoplasma diversity

MAGaa

MAGb

Product

Similarity (%)

MS/MSc 5632

MS/MSc PG2

Comments

MAGa1570

MAG1530

Type III R/M system:Methylase

75.3

+

+

 

MAGa1580

  

77.6

+

  

MAGa1770

MAG1790

DNA methylase

97.8

-

-

 

MAGa2070

MAG2070

DNA methylase

98.9

+

-

 

MAGa2700

MAG2550

Adenine-specific DNA methyltransferase

65.8

-d

-

Pseudogene in PG2

 

MAG2560

  

-

  

MAGa2710

MAG2570

Type II restriction endonuclease **

46.1

-

-

Pseudogene in PG2

 

MAG2580

  

-

  

No homolog

MAG3310

CpG DNA methylase

na

na

-

 

No homolog

MAG4030

Conserved hypothetical protein

na

na

-

BBH:Mmm SC - Putative C5 methylase (40%)

MAGa4470

MAG4250

Pseudogene of CpG DNA methylase (N-terminal)

83.4

-

-

 

MAGa4480

MAG4260

Pseudogene of CpG DNA methylase (C-terminal)

94.7

-

-

 

MAGa6280

MAG5640

Type I R/M system specificity subunit

75.0

-

+d

Locus hsd

MAGa6290

MAG5650

Modification (Methylase) protein of type I restriction-modification system HsdM

98.3

+

-

Locus hsd

MAGa6310

MAG5680

Type I R/M system specificity subunit

32.4

-

+d

Locus hsd

MAGa6330

MAG5700

HsdR, R/M enzyme subunitR

95.0

+

-

Pseudogenes in PG2

 

MAG5710

  

-

  

MAGa6340

MAG5720

Type I R/M system specificity subunit

30.9

+

-

Locus hsd

MAGa6350

MAG5730

Modification (Methylase) protein of type I restriction-modification system HsdM

90.3

+

+

Locus hsd

MAGa7650

MAG6680

Modification methylase

97.6

+

-

Modification methylase

MAGa3200 MAGa5050 MAGa6900

No homolog

CDSH

na

-

na

BBH: 92.0% with MCAP0297 - Mcap - adenine-specific DNA methylase

MAGa4250

No homolog

Modification methylase Bsp6I

na

+

na

BBH: 81.7% Bacillus sp. bsp6 IM Modification methylase Bsp6I

MAGa4260

No homolog

Type II restriction enzyme Bsp6I

na

+

na

BBH:55.1% Bacillus sp bsp6 IR Type II restriction enzyme Bsp6I

MAGa3950

No homolog

Cytosine-specific methyltransferase

na

+

na

BBH: Mmm SC MSC_0216 dcm Cytosine-specific DNA-methyltransferase Sau96I

MAGa3970

No homolog

Type II site-specific deoxyribonuclease, sau 96I-like

na

-

na

BBH: Mmm SC MSC_0215 sau 96I Type II site-specific deoxyribonuclease

  1. a, CDS of M. agalactiae strain 5632 (MolliGen Mnemonic).
  2. b, CDS of PG2 (MolliGen Mnemonic), pseudogenes are indicated in italic.
  3. c, Proteomic analyses (see materials and methods): (+) indicates that peptides were detected by MS/MS for the corresponding CDS, suggesting expression of the corresponding gene, (-) indicates that no specific peptides were detected for the corresponding CDS.
  4. d, only one peptide detected.
  5. e, MAG5640 and MAG5680 have common peptides.
  6. BBH, Best Blast Hit; Mmm SC, Mycoplasma mycoides subsp. Mycoides SC; Mcap, M. capricolum subsp. capricolum; na, not applicable; R/M, Restriction/Modification