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Figure 4 | BMC Genomics

Figure 4

From: Systematic overrepresentation of DNA termini and underrepresentation of subterminal regions among sequencing templates prepared from hydrodynamically sheared linear DNA molecules

Figure 4

Distribution of fragment sizes after shearing the circular and linear cosmid DNA. Aliquots of the sheared DNA samples used to construct the sequencing libraries were run on a 0.7% agarose gel alongside a 1 kb plus size marker. The gel was stained with SybrGold and scanned in a FluorImager. Molecular abundance was measured using an arbitrary value which was calculated by dividing the fluorescence intensity by molecular length. A) shows the resulting digital image. B) shows a graph of the size distributions for the three sheared DNA samples.

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BMC Genomics

ISSN: 1471-2164

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