Figure 2

Comparison of two TvBspA paralogues in isolate G3 and TvBspA orthologue between isolate G3 and SS-22. The two proteins TvBspA625 (TVAG_073760) and TvBspA805 (TVAG_154640) have the highest level of identity, 72%, between members of subfamily #13 (see text). (A) The TvBspA805 protein sequence with the TpLRR (green) and P/NRR (violet) aligned to compare individual repeats and their corresponding features in TvBspA625. The star indicates the first TpLRR that is part of the BlastP TvBspA625/TvBspA805 alignment (underlined segment) and that extends up to the C-termini. The inferred TMD is highlighted in yellow. (B) The corresponding TpLRR and P/NRR alignments for TvBspA625. The number of TpLRR is greater in TvBspA805, whereas the P/NRR is more extensive in TvBspA625. (C) Alignment comparing the C-terminal end of the TvBspA805 (G3 isolate: 805_G3) and TvBspA625 (G3 isolate: 625_G3, identical to sequence of isolate C-1:NIH, GenBank accession: AAM51159) proteins with the corresponding partial sequence of TvBsp625 derived from the clinical isolate SS-22 (625_SS-22, GenBank accession FJ881695). In TvBspA805 every second P/NRR (aligned in A) are underlined whereas for the TvBspA625 proteins every second P/NRR (aligned in B) are boxed. TvBspA625_SS-22 (7 P/NRR) has five fewer P/NRR compared to TvBspA625_G3 (12 P/NRR). The alignments of repeats were manually adjusted to maximise identity levels between repeats. Missing sequence data for the sequence from isolate SS-22 are indicated by question marks. The aligned sequences correspond to the segments between [----] in panels A and B. The sequences of the four peptides synthesized to generate mouse antisera and used to test antibody response in T. vaginalis infected patients are shaded in grey with differences highlighted in white (see text).