Analysis of deep sequencing microRNA expression profile from human embryonic stem cells derived mesenchymal stem cells reveals possible role of let-7 microRNA family in downstream targeting of Hepatic Nuclear Factor 4 Alpha

Table 2 Let 7 family of miRNAs are secreted by hES-MSC in large numbers. This table lists common transcripts in the extracellular samples that belong to the mid-range category of between 32 to 10,000 transcripts showing a predominance of let 7 family of miRNAs. It is interesting to note that no transcripts with counts greater than 10,000 were observed in the extracellular space.

Extracellular Transcript Number (Sample 1)	Extracellular Transcript Number (Sample 2)	Extracellular Transcript Number Sample 3	Transcript Sequence	miRNA Annotation
826	3653	3734	TGAGGTAGTAGATTGTATAGTT	hsa-let-7f
679	2748	1870	TGAGGTAGTAGTTTGTACAGTT	hsa-let-7g
287	69	331	AAGCTGCCAGTTGAAGAACTGT	hsa-miR-22
254	992	249	TGGAATGTAAAGAAGTATGTAT	hsa-miR-1
243	605	997	ACAGTAGTCTGCACATTGGTTA	hsa-miR-199a-3p
215	1225	931	TGAGGTAGTAGGTTGTATAGTT	hsa-let-7a
182	227	644	TAGCTTATCAGACTGATGTTGA	hsa-miR-21
170	100	515	TCAGTGCATGACAGAACTTGG	hsa-miR-152
164	389	834	TAGCACCATCTGAAATCGGTTA	hsa-miR-29a
112	42	160	TCCCTGAGACCCTAACTTGTGA	hsa-miR-125b
95	1051	149	TGAGGTAGTAGGTTGTATGGTT	hsa-let-7c
94	40	156	TGGCTCAGTTCAGCAGGAACAG	hsa-miR-24
87	159	100	AAAAGCTGGGTTGAGAGGGCGA	hsa-miR-320a
82	308	334	TGAGGTAGTAGTTTGTGCTGTT	hsa-let-7i
81	97	43	TGAGGGGCAGAGAGCGAGACTTT	hsa-miR-423-5p
71	55	310	TGGAGAGAAAGGCAGTTCCTGA	hsa-miR-185
66	46	256	AACCCGTAGATCCGAACTTGTG	hsa-miR-100
66	502	277	TGAGATGAAGCACTGTAGCTC	hsa-miR-143
39	104	87	AGAGGTAGTAGGTTGCATAGTT	hsa-let-7d
39	111	194	AGCTACATTGTCTGCTGGGTTTC	hsa-miR-221

ISSN: 1471-2164