Figure 6
From: Discovery of pathway biomarkers from coupled proteomics and systems biology methods
Schema of Methods First, proteins were prepared and subjected to LC/MS/MS analysis. Next, a permutation procedure was used to determine the p-value. Next, 4 previously published proteomic studies of breast cancer cell lines were used for comparison. Then, top networks and pathways were identified with Ingenuity Pathways analysis, KEGG and HPD pathway databases. And Level 2 and 5 of biological process in gene ontology are mainly studied. And then, two testing cancer dataset were used to validate the result. Last, assay development and clinical trials for panel biomarkers are planned for the future.