Table 6 Primer sets and real-time RT-PCR conditions used for analysis of selected H. irritan s ESTs.
From: Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758)
Unigene ID (Genbank accession number) | Gene description | Upstream/downstream primer sequences (5'-3') | PCR annealing conditions |
|---|---|---|---|
13_D07 (HO000820) | 5'-nucleotidase | AGTGGACAAATGTCCCGAAG AGCATTGGGGTTTGAATGAG | 55°C, 30 s |
6_F11 (HO000609) | T-cell immunomodulatory protein | CCGGTGACTTTGATGGAGAT GATAATGGCTCCCCTTTGGT | 55°C, 30 s |
10_G05 (HO000738) | RNase L inhibitor | GCCGATCGTGTTATTGTCCT CCGGATCGTTTTTGTTCAGT | 55°C, 30 s |
7_A04 (HO000619) | Proteasome subunit beta | CAGGCGAGGTCCATTATTGT AGTGCGCGACCTCAAGTAGT | 55°C, 30 s |
12_H09 (HO000808) | Proteasome maturation protein | GAGGAATCGTGAGGGTTTGA ACATGGGGTTGTCGGATAAA | 55°C, 30 s |
7_F08 (HO000644) | vATPase subunit d | TGTTTTTCCGTCACCAGTCA GGCACAAACCCTCCAAGTAA | 60°C, 30 s |
9_A08 (HO000679) | vATPase subunit f | TGTTGGATTCTTGCTTGGTG GGCACTGGTGATGTATGTGC | 60°C, 30 s |
17_H03 (HO001053) | vATPase proteolipid subunit | GTCCAGCCAGACTGTGATGA AATCAATCGCGGACAAAAAC | 60°C, 30 s |
26 (HO004524) | Ferritin light chain | TGATCATGTTGAACCCGAGA CGGCTGGTCAATTTCTTGAT | 60°C, 30 s |
154 (HO004652) | Ferritin heavy chain | GTTGTTGCCCCTGCTGTATT TGAAAAGTGGGCTCCCATAG | 60°C, 30 s |
84 (HO004582) | Ubiquitin-protein ligase | TCGCATCTGTTTGGATGTGT CGGGAAAACTTTTGAGTCCA | 60°C, 30 s |
146 (HO004644) | Ubiquitin family (UBQ-1) | CCCGACCAACAACGTTTAAT CGACGAAGACGGTGAATTTT | 60°C, 30 s |
4_E04 (HO000538) | Ubiquitin carboxyl-terminal hydrolase | AGCCAGAGATGTTGGAATGG TCGATGTAAATTGCCGCATA | 60°C, 30 s |
7 (HO004505) | Vitellogenin 3 | GAGCTTTTTGCGTTGTAGCC ACAAAAGTGGGAGCAACACC | 60°C, 30 s |
20 (HO004518) | Vitellogenin 1 | GAGCTTTTTGCGTTGTAGCC ACAAAAGTGGGAGCAACACC | 60°C, 30 s |
76 (HO004574) | Vitellogenin 2 | ACGGCCGGTTGTGAGATTAT AGCATCTTTTTCGGTCTTGC | 60°C, 30 s |
2_B12 (HO000479) | Elastase inhibitor | CAAGGGTGAATGGGAAAAGA TAAAGGCCTTCACGTTCCTG | 60°C, 30 s |
90 (HO004588) | Serine protease, midgut specific trypsin, secreted | TGCGTTATATTCCGTTGGTG CTTTGTCAACGGCATAAGCA | 60°C, 30 s |
230 (HO004728) | Serine protease | TGGCTACAATGAATGCAAGC GGTTAGCACCAGGGAACGTA | 60°C, 30 s |
(FJ025436) | H. irritans 16S rRNA | TTTAAATGGCCGCAGTATCC GATTTATAGGGTCTTCTCGTCTTTT | 60°C, 30 s |