Position and nt specific substitutions. The frequency of sequences with the indicated nt substitutions among total acquired, phred unfiltered sequences is plotted for 5C.C7 (A), OT-1 (B), and DO11.10 TCR (C). Mean+1 s.d. of 9 samples per lane is plotted for each of the 3 sequencing lanes to highlight lane-specific differences in position/nt error rates. Plotted lines are shown to aid visualization of results from single lanes and do not indicate continuity among x-axis variables.