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Table 3 Difference in mRNA levels between Fat (F) and Lean (L) and between G+ and G- muscles for genes chosen for quantification by real-time RT-PCR

From: Identification of differentially expressed genes in chickens differing in muscle glycogen content and meat quality

Symbol Name G+/G- F/L
   Microarray qRT-PCR Microarray qRT-PCR
Genes screened out from the microarray analyses     
ABHD5 Abhydrolase domain containing 5 1.367* 0.997 NS 1.527*
ACSL1 Acyl-CoA synthetase long-chain family member 1 1.391* 0.994 NS 0.765
CEBPB CCAAT/enhancer binding protein (C/EBP), beta 1.491* 3.266** NS 0.828
ETFA Electron-transfer-flavoprotein, alpha polypeptide 0.719* 1.036 0.896* 0.896
FOXO3 Forkhead box O3 1.329* 1.400 NS 1.146
LPAR1 Lysophosphatidic acid receptor 1 0.511* 0.472* NS 1.243
PDK4 Pyruvate dehydrogenase kinase, isozyme 4 0.645* 0.397† ND 3.007†
PIK3CD Phosphoinositide-3-kinase, catalytic, delta polypeptide 1.768* 0.856 NS 1.613
PPP1R12B Protein phosphatase 1, regulatory (inhibitor) subunit 12B 0.758* 0.776 NS 1.103
RGS2 Regulator of G-protein signaling 2 1.778* 2.433* 1.454* 0.821
RPIA Ribose 5-phosphate isomerase A 1.380* 0.945 NS 1.352
RPS6 Ribosomal protein S6 0.611* 0.678† NS 1.014
SRF Serum response factor (c-fos serum response element-binding transcription factor) 0.562* 0.405* 0.734* 0.734
UCP3 Uncoupling protein 3 (mitochondrial, proton carrier) 0.641* 0.601 NS 0.731
UGDH UDP-glucose dehydrogenase NS 0.503† 1.441* 1.477
UGP2 UDP-glucose pyrophosphorylase 2 1.469* 0.873 NS 0.898
Candidate genes chosen for targeted analyses by real-time RT-PCR §     
PRKAB1 AMP-activated, beta 1 non-catalytic subunit ND 0.603† ND 1.315
PRKAG2 AMP-activated, gamma 2 non-catalytic subunit ND 0.517* ND 1.129
PHKD Phosphorylase kinase, delta ND 0.683* ND 1.115
  1. For both microarray and real-time RT-PCR analyses, results are expressed as Fat to Lean and G+ to G- ratios of the expression gene.
  2. NS = non-significant; ND = not determined. Statistical significance is indicated as follows: †p value < 0.1, *p value < 0.05, **p value < 0.01.
  3. § Only genes identified as differentially expressed in at least one model are presented in the Table.