Figure 4

IFN-γ responses to early infection. UPEC or PBS were injected into rat kidney proximal tubules. A. Ratio of IFN-γ concentration at 8 h relative to 0 h, measured by ELISA, in serum from PBS or UPEC injected animals. * P-value <0.05 defined by a Student's t-test between PBS and UPEC injected animals log-transformed ratios of IFN-γ concentrations at 8 h to 0 h.B-D. Relative expression of different genes in spleen and kidney measured by qRT-PCR 8 h post-infection. Expression levels in UPEC (white bars) and PBS samples were normalized to that of PBS samples. * P-value <0.05 defined by Student's t-test between samples from PBS- and UPEC-injected animals. Gene expression in spleen tissue (B), kidney tissue that has been injected with UPEC or PBS (C), and naïve kidney tissue located ~1 cm from the injected site (D).In all panels, error bars show SD, n = 3.