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Table 7 RT-PCR confirmation of differences in gene expression: Growth/neurotrophic factor genes from Experiment 2.

From: Alteration of gene expression by alcohol exposure at early neurulation

  

ALC-NTC

ALC-NTO

  

Microarray

qRT-PCR

Microarray

qRT-PCR

Gene symbol

GenBank

Fold Change*

p-value*

Fold Change*

p-value*

Fold

p-value*

Fold Change*

p-value*

Ctgf

NM_010217

-1.7

0.01

-1.2

0.300

-2.2

0.004

-2.0

0.048

Edil3

NM146015

-1.6

0.00

-1.4

0.019

-1.9

0.025

Not tested

Efemp1

NM_146015

-1.5

0.01

-2.3

0.002

-2.3

0.001

-1.8

0.010

Igf1

NM_184052

-1.4

0.02

-2.6

0.001

-1.3

0.060

Not tested

Igfbp2

NM_008342

-2.0

0.02

-1.6

0.084

-1.5

0.035

-2.0

0.045

Ntf3

NM_008742

-1.2

0.30

-1.7

0.060

-1.8

0.030

-1.9

0.038

Tieg1

NM_013692

-1.2

0.36

-1.3

0.015

-1.5

0.010

-1.2

0.053

  1. *Control versus alcohol-treated
  2. Fold change: positive = ratio of alcohol-treated to control, negative = ratio of control to alcohol-treated. p-value: t-test of control vs. alcohol treated