Northern blot detection of three novel miRNAs, GPB125, GPB235 and GPB1131, from barley leaf tissues. U6 was used as loading control. Sizes of each miRNA and U6 are indicated. Uppercase P and GP represent the Pallas cultivar and the Golden Promise cultivar, respectively. GP also indicates Golden Promise plants grown under well-watered conditions. P (-p) represents Pallas plants grown in soil supplied with 22.5 mg phosphate (KH2PO4)/kg dry soil, while P (+p) represents Pallas plants grown in soil supplied with 75 mg phosphate (KH2PO4)/kg dry soil. GP (DREB3) represents Golden Promise plants transformed with the wheat DREB3 gene. GP (-water) represents Golden Promise plants grown under drought conditions (see Methods for details).