Figure 6

Tissue localization of the bovine ZNF280BY transcript in adult testis. The sense and antisense RNA of ZNF280BY are expressed in adult testis. A. The ZNF280BY sense RNA is expressed widely and evenly across all cell types in the seminiferous tubules. B. The antisense RNA of ZNF280BY was only detected in spermatids. Sense (A) and antisense (B) RNA of ZNF280BY were detected by the corresponding DIG-labeled cRNA probes. C. The bovine Protamine gene was used as the positive control, and there is no antisense mRNA of Protamine detected in the bovine testis [34]. D. The Haematoxylin and Eosin (H&E) staining was shown. Scale: bar = 200 μm.