Table 1 BAC libraries screening. cDNA probes used were either involved in the lignin biosynthetic pathway (PAL, C4 H, HCT, C3 H, CCoAOMT, CCR, F5H/CAld5H, CAD) or regulating this pathway (MYB1 and 2, RAC1).
Gene family | Gene | EMBL accession | library | Positive clones Array hybridization (a) | PCR (b) | Ratio (b/a) % |
|---|---|---|---|---|---|---|
Phenylalanine ammonia lyase (PAL) | EguPAL_ | CT987001 | EG__Ba | 15 | 11 | 63 |
Cinnamate 4-hydroxylase (C4H) | EguC4H | CT988030 | EG__Bb | 6 | 2 | 33 |
4 coumarate CoA ligase (4CL) | Egu4CL | AJ244010 | EG__Bb | 15 | 12 | 80 |
Hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase (HTC) | EguHTC | CT980202 | EG_Ba | 13 | 13 | 100 |
p-coumarate 3-hydroxylase (C3H) | EguC3H | CT986440 | EG__Bb | 5 | 3 | 60 |
Caffeoyl-CoA O- methyltransferase (CCoaOMT) | EguCCoaOMT | AF168778 | EG_Ba | 19 | 7 | 37 |
Cinnamoyl CoA reductase (CCR) | EguCCR | X79566 | EG_Ba | 13 | 10 | 77 |
Ferulate 5 hydroxylase/coniferaldehyde 5 hydroxylase (F5H/CAld5H) | EguF5H | CT987560 | Eg_Ba | 17 | 5 | 29 |
Caffeic acid/5- hydroxyoniferaldehyde O-methyltransferase (COMT) | EguCOMT | X74814 | EG__Bb | 19 | 12 | 63 |
Cinnamyl Alcohol dehydrogenase | EguCAD2 | X65631 | EG__Ba | 22 | 13 | 59 |
Rho-related small GTP-binding protein | EguRAC1 | DR410036 | EG__Bb | 33 | 3 | 9 |
R2R3 MYB transcription factor | EguMyb1 | AJ576024 | EB_Ba | 10 | 9 | 90 |
R2R3 MYB transcription factor | EguMyb2 | AJ576023 | EG__Bb | 7 | 6 | 86 |