mRNA expression levels of Class B, C, and F/S GPCRs. Microarray analysis of mRNA levels of GPCRs with known ligands for HEK293, AtT20, BV2 and N18 cell lines. A: Class B GPCRs. B: Class C GPCRs. C: Class F/S GPCRs. The gray lines indicate the threshold of statistical significance and the dark bars are proteins for which statistically significant levels of mRNA were detected. White bars indicate levels of mRNA for proteins that were present, but did not reach statistical significance. Abbreviations: BAI = Brain-specific angiogenesis inhibitor; CELSR = Cadherin EGF LAG seven-pass G-type receptor; CRF = Corticotropin-releasing factor; ND = Not determined; PACAP = pituitary adenylyl cyclase-activating polypeptide; VIP = Vasoactive intestinal peptide.