Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transition

Tadiso, Tariku Markos; Krasnov, Aleksei; Skugor, Stanko; Afanasyev, Sergey; Hordvik, Ivar; Nilsen, Frank

doi:10.1186/1471-2164-12-141

Table 2 Primer/ probe list

From: Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transition

S.N	Sequence Definition	Code		Primer/ probe (5'-3')	Product length	GenBank Accession (GI)
1.	Apoptosis regulator Bcl-X	Apo	Fwd	TACTAAGTGTTGCCGTTGA	109	209734267
			Rev	TAATCCAATCTGTGTCATTCTG
2.	Beta-2-microglobulin precursor	B2M	Fwd	CACAGACAGACACAGACA	102	221220497
			Rev	CAACGATTGACAGAATAGACTT
3.	Carboxypeptidase A1 precursor	CarPep	Fwd	AGCATACCAAGGACAACAC	75	209732661
			Rev	TACAACAGTACAATGACACAGT
4.	Chymotrypsin B	ChyTry	Fwd	CTGTCCATACTGTATATTGCTAT	111	209734305
			Rev	GCTATAATGCTTAGGTGTTGTA
5.	Collagenase 3 precursor	COL3	Fwd	ATCTGTGCTTACTACTAATCAAC	81	209156091
			Rev	GGGCTTCATCTTCTTTACTG
6.	Complement C1q subcomponent subunit B precursor	C1qB	Fwd	CTGTCTGTCGTTGTCTTC	89	223649475
			Rev	ATGGTCTGTTGGTCTGTA
7.	Elastase-1	Ela	Fwd	ACCGTCAACAAAGTCTTCA	75	S31963336
		Rev		CAGCAGAGCGATGTCATA
8.	Fish virus induced TRIM protein	TRIM	Fwd	GCATGGCACAATAATAACT	75	S35697379
			Rev	GTCCAGATACACTCCTAC
9.	Trypsin-2	Try2	Fwd	CAGTTGTCGGTTGAGATG	81	S18845530
			Rev	CAAGATGTGCCAGATAGC
10	Trypsin-3	Try3	Fwd	CATTATTCTTCTCGCTCTG	81	S31964271
			Rev	TCATACCCTCCAACAATC
11	Tumor necrosis factor alpha	TNFα	Fwd	ACAAAGAGGGCCAGGGATTC	100	126507266
			Rev	GAGGCCTGGCTGTAGACGAA
12	Interleukin-1 receptor-like protein	IL1R1	Fwd	AGCAGGATGTCCTCGGTCTA	202	185136290
			Rev	TGGGTAGCGGTGTAGTTTCC
13	Interleukin-1 beta	IL1β	Fwd	ACCGAGTTCAAGGACAAGGA	196	186288127
			Rev	GCAGCTCCATAGCCTCACTC
14	Gamma-interferon-inducible lysosomal thiol reductase	GIILTR	Fwd	CTATGTGCCTTGGATTGT	79	209732609
			Rev	CAGAGTGAAGAGTGAAGAC
15	HSP70_ONCMY Heat shock cognate 70 kDa protein	HSP70	Fwd	TCACTAGAGTCCTATGCT	85	CL7Contig1
			Rev	TTGTCTTGTCCTCATCAC
16	Lipopolysaccharide-induced TNF-a factor homolog	LPSi-TNFα	Fwd	CAATTCCTTCGACCTCAT	85	209734201
			Rev	GCTCTTCTCCATACTGTC
17	Metalloreductase STEAP4	STEAP4	Fwd	CTCCAACTCTGAAGACTATT	103	S48396453
			Rev	GAGCACTGTCAATCAATG
18	Myosin, heavy polypeptide 9, non-muscle	Myo	Fwd	GCAGTTGAGACTCTACAGTGGAA	75	CB498954
			Rev	ACAGCGTGTTGAGTGTGGTT
19	Programmed cell death protein 2	PCDP	Fwd	GCATAGACAGCCACAATC	81	209735097
			Rev	GAGCGTAACAACCTGAAG
20	Prostaglandin E synthase 3	PGES	Fwd	TCCAGCCAATGTCTTAGT	99	223672934
			Rev	AAGCACGGTATAACTGAAC
21	P-selectin precursor	P-sel	Fwd	CTGGTGATTCTATTGATGAC	86	209154193
		Rev		TTGACGCTGTAGTTGTAT
22	CD8α	CD8α	Fwd	CGTCTACAGCTGTGCATCAATCAA	118	185135177
			Rev	GGCTGTGGTCATTGGTGTAGTC
23	Interleukin-12	IL-12	Fwd	TCTACCTACACGACATTGTCCAGCC	62	209736091
			Rev	ATCCATCACCTGGCACTTCATCC
24	Prostaglandin D synthase	PGDS	Fwd	ATCCCAGGCCGCTTCAC	59	304376917
			Rev	ACACGCATGTCATTTTCATTGTT
			Probe	TTCACCAGCCAGCGTT
25	Matrix metalloproteinase-13	MMP13	Fwd	GCCAGCGGAGCAGGAA	56	213514499
			Rev	AGTCACCTGGAGGCCAAAGA
			Probe	TCAGCGAGATGCAAAG
26	T-cell receptor alpha	TCRα	Fwd	GACAGCTACTACAGCCAGGTT		209736003
			Rev	CAGAATGGTCAGGGATAGGAAGTT
			Probe	ACACAGATGCAAAGATC
27	CD3ε	CD3ε	Fwd	TCAGGGCTCGGAAGAAGTCT	68	185135943
			Rev	GCCACGGCCTGCTGA
			Probe	CCAAAAACCCACTTCCC
28	CD4-like protein, variant 1	CD4-1	Fwd	GAATCTGCCGCTGCAAAGAC	75	185135736
			Rev	AGGGATTCCGGTCTGTATGATATCT
			Probe	CCCAAACCAAAAGGATTC
29	CD8β	CD8β	Fwd	GGAGGCCAGGAGTTCTTCTC	70	185135192
			Rev	GGCTTGGGCTTCGTGACA
			Probe	ACCCGGAGAAACTC
30	MHC class I antigen	MHC-I	Fwd	CAACGCCACAGGCAGTCA	64	25573077
			Rev	CGGTACTCATTCTGAGCTGTGTTAC
			Probe	CACCAAACTCAAGTGGG
31	MHC class II antigen	MHC-II	Fwd	CTCACTGAGCCCATGGTGTAT	117	223672978
			Rev	GAGTCCTGCCAAGGCTAAGATG
			Probe	CTGGGACCCGTCCCTG
32	Immunoglobulin mu	IgM	Fwd	TGAGGAGAACTGTGGGCTACACT	69	2182101
			Rev	TGTTAATGACCACTGAATGTGCAT
			Probe	CATCAGATGCAGGTCC
33	Immunoglobulin tau	IgT	Fwd	CAACACTGACTGGAACAACAAGGT	97	260766539
			Rev	CGTCAGCGGTTCTGTTTTGGA
			Probe	AGTACAGCTGTGTGGTGCA
34	Elongation factor 1A	EF1A	Fwd	CCCCTCCAGGACGTTTACAAA	57	224587629
			Rev	CACACGGCCCACAGGTACA
			Probe	ATCGGTGGTATTGGAAC

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ISSN: 1471-2164

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