Figure 5

miRNA expression measurements by Fluidigm dynamic array and Affymetrix microarray. The raw intensity values of microarray data were transformed to log₂ values for comparison to PCR Ct values generated by the qPCR platform. Gene expression differences between FF and FFPE were compared against 59 shared genes. Fold differences by the dynamic array (log₂) were calculated by ΔΔCt method; ΔCt = (target miRNA log₂ values-hsa-mir-16 log₂ value), ΔΔCt = (fresh frozen ΔCt-FFPE ΔCt).