Table 1 Comparison of gene expression measurements between Affymetrix microarray and Fluidigm 96.96 dynamic array.
From: Quantitative miRNA Expression Analysis Using Fluidigm Microfluidics Dynamic Arrays
Platform | Sample†| Background | Signal to Noise | Positive Signal Intensity or Ct | Detection Rate (%) | Replicate Correlation | |||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | AVE | MAX | Â | MIN | MAX | AVE | Â | Â |
Microarray (847 miRNA) | FF#1a | 2.66 | 15.79 | 189 | 3.49 | 10530 | 503 | 33 | 0.997 |
| Â | FF#1b | 2.66 | 11.68 | 185 | 1.73 | 10313 | 493 | 33 | Â |
| Â | FFPE#9a | 2.26 | 35.38 | 208 | 2.34 | 9331 | 470 | 35 | 0.996 |
| Â | FFPE#9b | 2.22 | 17.85 | 209 | 2.59 | 8724 | 464 | 35 | Â |
96.96 dynamic array (94 miRNA) | FF#1a | N/A | N/A | - | 7.9 | 22.4 | 16.7 | 91 | 0.954 |
| Â | FF#1b | N/A | N/A | - | 5.0 | 22.7 | 16.2 | 91 | Â |
| Â | FFPE#9a | N/A | N/A | - | 5.9 | 35.9 | 19.5 | 85 | 0.956 |
| Â | FFPE#9b | N/A | N/A | - | 4.2 | 34.9 | 19.0 | 85 | Â |