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Table 4 Enzymes involved in fatty acid biosynthesis and metabolism identified by annotation of the D. tertiolecta transcriptome

From: Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

Enzyme Symbol EC Number Number of transcripts 1%Sequence alignment with corresponding enzymes in model organisms (Accession #)
     C. reinhardtii V. carteri
Fatty acid biosynthesis      
Biotin carboxylase BC 6.3.4.14 3 2NM 46 (EFJ41621.1)
Acetyl-CoA carboxylase ACC 6.4.1.2 7 28 (XP_001700442.1) 28 (EFJ46223.1)
malonyl-CoA-ACP transacylase MAT 2.3.1.39 4 NM 62 (EFJ49352.1)
Beta-ketoacyl-ACP synthase I KAS I 2.3.1.41 7 NM NM
Beta-ketoacyl-ACP synthase II KAS II 2.3.1.179 12 NM NM
Beta-ketoacyl-ACP synthase III KAS III 2.3.1.180 1 NM NM
Beta-ketoacyl-ACP reductase KAR 1.1.1.100 7 63 (XP_001703473.1) 61 (EFJ52014.1)
3R-hydroxyacyl-ACP dehydrase HAD 4.2.1.- 2 NM NM
Enoyl-ACP reductase (NADH) EAR 1.3.1.9 5 NM NM
Oleoyl-ACP thioesterase OAT 3.1.2.14 1 NM NM
Acyl-ACP thioesterase A FatA 3.1.2.14, 3.1.2.- 1 NM NM
Acyl-ACP thioesterase B FatB 3.1.2.14, 3.1.2.- 0 NM NM
Fatty acid desaturation      
Δ9 Acyl-ACP desaturase AAD 1.14.19.2 5 76 (XP_001691597.1) 76 (EFJ49192.1)
Δ126)-Desaturase Δ12D 1.14.19.6 3 54 (XP_001691669.1), 47 XP_001693068.1) 54 (XP_002955859.1), 47 (XP_002949932.1)
Δ153)-Desaturase Δ15-D 1.14.19.- 2 NM NM
Fatty acid elongation      
3-Hydroxyacyl-CoA dehydrogenase CHAD 1.1.1.35 9 NM NM
Long-chain-3-hydroxyacyl-CoA dehydrogenase LCHAD 1.1.1.211 2 NM NM
Enoyl-CoA hydratase ECH 4.2.1.17 16 NM 70 (EFJ49010.1)
Trans-2-enoyl-CoA reductase (NADPH) TER 1.3.1.38 3 90 (XP_001690095.1) NM
Palmitoyl-CoA hydrolase PCH 3.1.2.22 1 19 (XP_001698944.1) 19 (EFJ40725.1)
Fatty acid catabolism      
Long-chain acyl-CoA synthetase ACSL 6.2.1.3 3 77 (XP_001693692.1) 77 (EFJ51208.1)
Acyl-CoA oxidase AOx 1.3.3.6 2 39 (XP_001699193.1) NM
Acyl-CoA dehydrogenase ACADM 1.3.99.3 16 45 (XP_001699193.1), 84 (XP_001693484.1), 73 (XP_001695945.1) NM
Enoyl-CoA hydratase ECH 4.2.1.17 16 NM 70 (EFJ49010.1)
Long-chain 3-hydroxyacyl-CoA dehydrogenase LCHAD 1.1.1.211 2 NM NM
Acetyl-CoA acyltransferase ACAT 2.3.1.16 1 NM NM
3-hydroxyacyl-CoA dehydrogenase CHAD 1.1.1.35 9 NM NM
Acetyl-CoA C-acetyltransferase thiL 2.3.1.9 6 39 (XP_001694888.1) 34 (EFJ47048.1), 34 (EFJ39622.1), 34 (EFJ46961.1)
3-hydroxybutyryl-CoA epimerase   5.1.2.3 8 NM NM
Enoyl-CoA isomerase ECI 5.3.3.8 8 NM NM
Alcohol dehydrogenase ADH 1.1.1.1 3 80 (XP_001693934.1) 53 (EFJ45245.1)
Aldehyde dehydrogenase (NAD+) ALDH 1.2.1.3 3 24 (XP_001696928.1) 21 (EFJ40853.1)
Ferredoxin-NAD+ reductase FNR 1.18.1.3 1 NM NM
  1. 1In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.
  2. 2NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.