Figure 2From: RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalizationStandard deviation of commonly used and novel reference genes. Standard deviation (SD) of gene expression of commonly used reference genes (A) across all samples (a) or across subsets of tissue-specific samples (b) from the AffymetrixMouse430 2.0 array dataset. In (B), for each subset of tissue-specific samples, the most stable genes were identified using Genevestigator RefGenes. Their respective expression SD across all arrays (c) or across subsets of tissue-specific arrays (d) is shown. The control reference genes used in this study and shown in plot A were: HSP90AB1, TFRC, B2 M, NONO, GUSB, UBC, ACTB, H2AFZ, POLR2A, TUBB4, HIST2H2AA1, RPL22, GAPDH, YWHAZ, CANX, CYC1, SDHA, EIF4A2, ATP5B, and EEF1E1.Back to article page