Figure 1

Sheep Bone Healing Model. A Standard bone healing was investigated in a 3 mm tibial osteotomy model stabilized with a medially mounted rigid external fixator (left). Delayed healing was investigated in a 3 mm tibial osteotomy model stabilized with a medially mounted rotationally unstable (right) external fixator (caudo-lateral view). B Cranio-caudal radiographs of the rigid (left) and unstable (right) fixator group 63 days post-operation. Images modified from Schell et al. 2008 [17]. C Photomicrographs of representative histological sections of the periosteal bony callus at 14 days from standard healing. Newly formed bone in the periosteal callus covered by a layer of osteoid seam (asterisks, left image) and region of intramembranous bone formation (right image) with mineralization of deposited osteoid (asterisks). D Photomicrographs of representative histological sections of the periosteal bony callus at 14 days from delayed healing. Increased amount of osteoid (asterisks) covering the newly formed bone (left image) and less mineralization of deposited osteoid (right image). (C, D) Movat Pentachrome staining (left column), Safranin-Orange/von Kossa staining (right column). MdB: mineralized bone. The scale bars in lower right hand corners are equal to 100 μ m.