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Table 1 Changes in the relative abundance of proteins in T. pseudonana exposed to benzo(a)pyrene

From: Proteomic analysis of the marine diatom Thalassiosira pseudonana upon exposure to benzo(a)pyrene

Regulated proteins based on two or more peptides at >95% confidence

Name

Accessiona

Gene name

GO

Peptides

(95%)b

Regulation

Factorc

t-test

C-5 cytosine-specific DNA methylase, P

B8BVP1

THAPSDRAFT_21517

Metabolic process

4

2.30

0.045

Mitochondrial chaperonin

B5YLQ5d

HSP60

Chaperone

7

1.48

0.018

Cysteine synthase

B8BUL0

THAPSDRAFT_270338

Biosynthetic process

2

1.30

0.003

N-acetylornithine aminotransferase

B8CGH5d

THAPSDRAFT_270136

Metabolic process

3

1.28

0.004

3-isopropylmalate dehydrogenase

B8C2I2

THAPSDRAFT_268875

Oxidation reduction

3

1.21

0.001

Proteasome subunit alpha type

B8C0E7d

PSA4

Protein catabolism

2

1.21

0.032

Fucoxanthin chl a/c light-harvesting protein

B8C2Y4

Lhcr10

Photosynthesis

2

0.76

0.021

Eukaryotic translation initiation factor 4A

B8CC33

THAPSDRAFT_9716

Translation

2

0.68

0.041

CPSase

B8BZG0

THAPSDRAFT_40323

Metabolic process

5

0.63

0.007

Predicted protein

B8C0M3

THAPSDRAFT_22483

Unassigned

2

0.55

0.041

Predicted long chain acyl CoA synthetase

B8CDL6

THAPSDRAFT_25042

Metabolic process

2

0.53

0.050

Fucoxanthin chl a/c light-harvesting protein

B8C0K3

Lhcr4

Photosynthesis

2

0.50

0.003

Adenine nucleotide translocator

B8BR30

ANT1

Membrane transport

3

0.45

0.049

Potentially regulated proteins based on a single peptide at >95% confidence and additional peptides with lower confidence

Name

Accession

Gene name

GO

Peptides

(95%)

Regulation

factor

t-test

Dihydrodipicolinate reductase

B8C5E0

THAPSDRAFT_28544

Oxidation reduction

1

1.67

0.030

Predicted NonF-related protein; DJ-1/PfpI family

B5YN08d

THAPS_6979

Unassigned

1

1.21

0.016

Inorganic pyrophosphatase

B8C6T9

THAPSDRAFT_269348

Metabolic process

1

1.21

0.030

Porin/voltage-dependent anion-selective channel

B8BQH4

THAPSDRAFT_25918

Membrane transport

1

0.80

0.043

Silicon transporter

Q0QVM8d

SIT1

Membrane transport

1

0.78

0.013

Rubisco expression protein

B8LEU2

cfxX

Photosynthesis

1

0.57

0.041

  1. a Accession numbers, gene name and gene ontology information of proteins taken from the UniProtKB/Swiss-Prot database
  2. b Values indicate the number of peptides used for identification and quantification of the proteins.
  3. c Protein ratios in three biological replicates with 0.8 > Regulation factor < 1.2 and P-values <0.05
  4. d Proteins showing the same regulation trend in the DNA microarray experiments