Gene discovery in EST sequences from the wheat leaf rust fungus Puccinia triticina sexual spores, asexual spores and haustoria, compared to other rust and corn smut fungi

Table 1 cDNA libraries produced and number of generated quality EST reads

cDNA lib	source	designation ^a	# reads	unigenes ^b	singlets	contigs	predicted fungal ^c	stage-specific ^d
PT029	aeciospores	pte	1292	208 (16%)	166	42	199 (95%)	100 (48%)
PT030	pycniospores	ptp	4869	1252 (26%)	807	445	1116 (89%)	961 (77%)
PT031	dormant teliospores	ptt	3703	1165 (31%)	878	287	1041 (89%)	801 (69%)
PT033	isolated haustoria	ptih	4896	2886 (59%)	2620	266	307 (11%)	441 (15%)
PTDH	isolated haustoria	ptih	n/a	1921	354	1567	1236 (64%)	1069 (56%)
PTDG	germinated urediniospores	ptg	n/a	1003	83	920	740 (74%)	398 (40%)

^a life cycle stage-specific suffix, used at the end of all EST sequences: pte, P uccinia t riticina ae ciospores; ptp, pycniospores; ptt, teliospores; ptih, isolated haustoria; ptg, germinated urediniospores.
^b non redundant sequences obtained via CAP3 analysis of life cycle stage-specific EST set (as percent of reads generated), yielding singlets and contigs
^c number of unigenes predicted to be fungal (as percent of compiled unigenes for that stage)
^d number of unigenes not represented in other stages (as percent of all unigenes from this library)

ISSN: 1471-2164