Table 3 Reliability examination of the randomly selected 30 feeding Ahringer C. elegans RNAi bacterial strains with different scores

1

100

F18H3.3

X-6I10

√

F18H3.3

X-6I10

√

√

2

100

F22E12.4

V-7A24

√

F22E12.4

V-7A24

√

√

3

100

T07D1.4

X-1J16

√

T07D1.4

X-1J16

√

√

4

100

K09G1.4

V-6P21

√

K09G1.4

V-6P21

√

√

5

100

T26G10.1

III-5C07

√

T26G10.1

III-5C07

√

√

6

100

W01C9.5

II-6A04

√

W01C9.5

II-6A04

√

√

7

49

F36H12.3

IV-2B04

√

F36H12.17

IV-2D04

×

√

Remapped. Probably sample loading error: position of the strain was shifted vertically for two rows in same column of the same plate.

8

48

C17C3.13

II-4D02

√

C17C3.13

II-4D02

√

√

9

48

M142.1

III-5F06

√

F54F12.2

III-6D13

√

√

Remapped. Nonspecific amplicon and may be prevented by MFEprimer evaluation on the primer set at first.

10

46

T08D2.2

V-13E02

√

W07B8.1

NA (not in the library)

√

√

Remapped. Nonspecific amplicon and may be prevented by MFEprimer evaluation on the primer set at first.

11

44

T17A3.3

III-1E13

√

NA

NA

×

×

Empty vector construction based on direct sequencing

12

42

F58B4.1

V-7B19

√

F58B4.1

V-7B19

√

√

13

36

F48G7.5

V-1G12

√

F48G7.5

V-1G12

√

√

14

32

F32B5.4

I-1I14

√

C45E1.1

I-1M14

×

√

Remapped. Probably sample loading error: position of the strain was shifted vertically for four rows in same column of the same plate.

15

30

K11H12.1

IV-1A12

√

K11H12.1

IV-1A12

√

√

16

28

C03H5.5

II-1O03

√

Y48G8AL.11

NA (not in the library)

√

√

Remapped. Nonspecific amplicon and may be prevented by MFEprimer evaluation on the primer set at first.

17

27

C32E8.3

I-1H21

√

C32E8.3

I-1H21

√

√

18

26

F10G8.2

I-5C13

√

F10G8.2

I-5C13

√

√

19

24

C14B1.10

III-1J10

√

NA

NA

√

×

Nonspecific amplicon (Intergenic region [438973..440037] in chromosome I) and may be prevented by MFEprimer evaluation on the primer set at first.

20

23

F59H6.8

II-2E10

√

F59H6.8

II-2E10

√

√

21

22

R09A1.2

V-1F13

√

NA

NA

√

×

Nonspecific amplicon (Intergenic region [3064499..3065944] in chromosome V) and may be prevented by MFEprimer evaluation on the primer set at first.

22

22

B0554.1

V-1K09

√

Y39D8A.1

V-1K07

×

√

Remapped. Probably sample loading error by the manufacture: position of the strain was shifted horizontally for two columns in same row of the same plate.

23

20

C45G7.5

IV-1N13

√

C45G7.5

IV-1N13

√

√

24

14

W02G9.1

V-2M18

√

W02G9.1

V-2M18

√

√

25

4

C49A1.1

I-7I17

√

Y48G8AL.13

NA (not in the library)

√

√

Remapped. Nonspecific amplicon and may be prevented by MFEprimer evaluation on the primer set at first.

26

0

Y110A2A_4093.a

II-9H01

×

K02F6.9

NA (not in the library)

√

√

Retired gene name and remapped to the new gene.

27

0

C38H2.2

III-5F21

×

NA

NA

×

×

Nonspecific amplicon (Located in chromosome X according to the BLAST similarity search) and may be prevented by MFEprimer evaluation on the primer set at first.

28

0

Y119D3_462.c

III-7A23

×

Y119D3A.2

NA (not in the library)

√

√

Retired gene name and remapped to the new gene.

29

0

Y55B1B_119.g

III-7G04

×

Multiple targets predicted

NA

×

√

Retired gene name and remapped to multiple new genes.

30

0

Y67D8A_349.a

IV-8B22

×

NA

NA

×

×

Retired