Deep sequencing analysis of the developing mouse brain reveals a novel microRNA

BMC Genomics

Table 1 Annotation of unique tags

	Unique tags
Annotation of unique tags	22 nt of 3' end*^@	22 nt of 5' end*^@	Combined non-redundant^	Total combined counts^	Additional file(s)^#
Repetitive elements	4,651	3,266	7,136	234,381	2 and 3
'Infrastructure' non-coding RNAs	6,907	30	6,929	17,853	2 and 3
miRNAs, miRNA stars and pre-miRNAs	45,623	0	45,623	1,867,113	4
RefSeq	2,431	22	2,448	2,775	5 and 6
Redundant mouse ESTs	5,954	737	6,584	48,465	7 and 8
Unique locus in the genome without annotation	1,377	439	1,752	7,656	9 and 10
Multiple loci in the genome without annotation	3,761	241	3,821	6,039	11 and 12
Total	70,704	4,735	74,293	2,184,282

* No mismatch was allowed during Bowtie analysis.
^ Combined non-redundant values.
^@ Redundant values are presented. Redundant values were due to the same unique tag being analysed twice in both 5' and 3' Bowtie analysis.
^# Annotation of unique tags based on 3' or 5' end sequences are presented in the additional files 2, 4, 5, 7, 9, 11 and 3, 6, 8, 10, 12, respectively.

ISSN: 1471-2164