Figure 1

Evolutionary relationship of S-LAPs. (A) Phylogeny of D. melanogaster S-LAPs and granny-smith. Minimum Evolution distance methods were used to construct the bootstrap consensus protein phylogeny (1000 replicates). Bootstrap values are presented next to relevant nodes and the phylogeny is drawn to scale based on amino acid substitutions per site. This analysis revealed two related clusters of D. melanogaster S-LAPS, Cluster I and II. (B) Expanded S-LAP family in Drosophila. Comparative genomic analyses identified a total of 17 related aminopeptidases in A. gambiae, C. quinquefasciatus, A. aegypti, T. castaneum, A. pisum and A. mellifera. Minimum evolution distance methods were used to construct the bootstrap consensus protein phylogeny (1000 replicates). Bootstrap values are presented next to relevant nodes and the phylogeny is drawn to scale based on amino acid substitutions per site. S-LAP Cluster I, comprised of 6 genes in Drosophila, is related to a single copy aminopeptidase in mosquitos and T. castaneum. Similarly S-LAP Cluster II, comprised of two genes in Drosophila, is related to a single copy aminopeptidase in other insect taxa with the exception of C. quinquefasciatus where two gene copies are present in the genome. In contrast, granny-smith has "one-to-one" orthology relationships in other insect taxa.