Figure 2

A schematic illustration of a BAC fingerprinting contig anchored to the rainbow trout Chr. 2 using microsatellites isolated from BACs. The four microsatellite markers from Ctg260 (224 clones; 1,584 CB or approximately 2.7 Mb) were mapped to Chr. 2 and the TAP1 positive BACs (highlighted in green) were previously identified by probe hybridization and confirmed by PCR and direct sequencing. The microsatellites order shown is based on the FPC map (not the genetic map). Markers in bold blue (OMY4005 and 4006) were localized on the linkage group at LOD4 and markers in regular font at LOD0. The genetic distance between the LOD4 markers is marked by a solid-line arrow and between markers that were localized at lower confidence by broken-line arrows.