Expression of the scavenger receptor, CD163, and CD68 in lung macrophages from WT, 4L, and 9 V/null mice. Anti-CD68 is green (FITC) and anti-CD163 is red (Alexa-610). The colocalization (yellow) of CD163 signals with CD68 in 9 V/null lungs showed about half the storage cells were double positive and no cells staining only with CD163. Some low level signals for CD68 and CD163 were seen in small cells in 4L lungs. The scale bars are 20 μm. Magnification = 400×.