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Table 3 RT-qPCR of the differentially expressed genes between resistant and susceptible lines

From: Transcriptomic analysis of milk somatic cells in mastitis resistant and susceptible sheep upon challenge with Staphylococcus epidermidis and Staphylococcus aureus

Microarray result Genes All data Sa data Se data
   Resistant Susceptible Resistant Susceptible Resistant Susceptible
  CRYL1 26.50 ± 29.98** 4.65 ± 5.68 40.39 ± 37.92** 6.20 ± 6.66 12.61 ± 8.71* 3.10 ± 4.57
  TP53    4.53 ± 3.51** 1.16 ± 0.72   
  RARα 1.34 ± 0.82** 0.76 ± 0.37 0.95 ± 0.58* 0.51 ± 0.31 1.72 ± 0.90* 1.02 ± 0.20
  SLC40A1    5.07 ± 8.07* 1.29 ± 1.10   
Main List GTPBP4    0.59 ± 0.47* 1.21 ± 0.92   
  TMEM87B    0.73 ± 0.14* 1.19 ± 0.86   
  PPAPDC1B 0.43 ± 0.39 0.81 ± 0.92 0.25 ± 0.13 0.26 ± 0.19 0.61 ± 0.50* 1.36 ± 1.05
  EIF4EBP1 1.54 ± 0.80 1.27 ± 0.75 1.93 ± 0.75 1.49 ± 1.01 1.14 ± 0.67 1.05 ± 0.33
  MAPRE1    0.87 ± 0.57 1.16 ± 0.76   
  SAA2    0.33 ± 0.31** 1.86 ± 2.30   
  ITGB6    0.42 ± 0.31** 1.49 ± 1.49   
Sa list S100A2    0.58 ± 0.39* 1.28 ± 0.94   
  CCL5    0.55 ± 0.36* 1.35 ± 1.02   
  TLR2    2.04 ± 2.17 1.09 ± 0.47   
  CAPN3 3.63 ± 2.71** 1.52 ± 0.98 4.07 ± 3.23 1.66 ± 0.81 3.19 ± 2.28* 1.38 ± 1.18
Se list PSMD4 0.82 ± 0.28** 1.06 ± 0.31 0.96 ± 0.27 1.08 ± 0.33 0.67 ± 0.22** 1.04 ± 0.32
  ST3GAL4 1.56 ± 1.31** 0.82 ± 0.50 0.96 ± 0.73 0.54 ± 0.37 2.16 ± 1.55** 1.10 ± 0.48
  1. The results represent the mean ± standard deviation of the relative expression in qPCR of seventeen differentially expressed genes identified in the microarray analyses with all data (main list), S. aureus (Sa list) or S. epidermidis data (Se list). qPCR were performed with Sa or Se samples or with both Sa an Se samples (All data). A non parametric Wilcoxon test was performed with SAS to identify the differentially expressed genes. ** p-value < 0.05; * p-value < 0.10.